An all-in-one sterile device for accurate and convenient dilutions. Features a loop attached to a reservoir where patented Snap-Valve™ technology contains 1.0 mL of buffer. Simply collect sample, break the Snap-Valve, and a dilution is immediately ready to be poured onto dry media film or streaked onto an agar plate. Allows quicker and more convenient dilutions without special lab equipment. Aseptically filled and gamma irradiated. No need to prepare dilution buffers. Eliminates the need for pipettes. Reduces labor and lab equipment expense. 12 month shelf life at refrigerated temperatures.